Top: single frame cortical F-actin consists of irregularly sized patches, which, as illustrated in the kymograph made from the outlined region (bottom) travel continually across the surface. (b) High magnification surface view of activated frog egg expressing GFP-UtrCH. Bottom: In the kymograph, F-actin waves create slanted bands with semi-regular spacing. Top: single frame cortical F-actin consists of irregularly sized patches throughout cortex, which, as illustrated in the kymograph made from the outlined region (bottom) travel continually across the surface. ( a) Surface view of frog blastomeres expressing GFP-lifeact to label F-actin. Utilization of Arg-elution method for FLAG-tag based chromatography. Anillin regulates cell-cell junction integrity by organizing junctional accumulation of Rho-GTP and actomyosin. Imaging the cytoskeleton in live Xenopus laevis embryos. Excitable actin dynamics in lamellipodial protrusion and retraction. Regimes of wave type patterning driven by refractory actin feedback: transition from static polarization to dynamic wave behaviour. Rho activation is apically restricted by Arhgap1 in neural crest cells and drives epithelial-to-mesenchymal transition. Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo. Reassembly of contractile actin cortex in cell blebs. A Rho GTPase signal treadmill backs a contractile array. A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus. Mitotic trigger waves and the spatial coordination of the Xenopus cell cycle. The M-phase-promoting factor modulates the sensitivity of the Ca 2+ stores to inositol 1,4,5-trisphosphate via the actin cytoskeleton. The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm. Duration of stimulus and latent periods preceeding furrow formation in sand dollar eggs. A microtubule-dependent zone of active RhoA during cleavage plane specification.
ATP-site directed inhibitors of cyclin-dependent kinases. Cyclin synthesis drives the early embryonic cell cycle. Electrical turbulence in three-dimensional heart muscle. Phase geometries of two-dimensional excitable waves govern self-organized morphodynamics of amoeboid cells. Dynamics of Cdc42 network embodies a Turing-type mechanism of yeast cell polarity. Traveling waves in actin dynamics and cell motility. Self-organization of the phosphatidylinositol lipids signaling system for random cell migration. Modeling the self-organized phosphatidylinositol lipid signaling system in chemotactic cells using quantitative image analysis. Spiral calcium wave propagation and annihilation in Xenopus laevis oocytes. The myriad roles of Anillin during cytokinesis. Concentric zones of active RhoA and Cdc42 around single cell wounds. Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish. Lifeact: a versatile marker to visualize F-actin. Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
An actin-based wave generator organizes cell motility. Regulation of cytokinesis by Rho GTPase flux. The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle. Transitions regulating the timing of cytokinesis in embryonic cells. Cytokinesis in Animal Cells (Cambridge Univ. An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos. Rho GTPase activity zones and transient contractile arrays. I use a very simple technique, which requires just a few lines of code.Bement, W.